jak inhibitors  (Innovative Therapies)

Journal: bioRxiv

Article Title: PTPN1/2 inhibits alveolar macrophage-mediated control of lung metastasis

doi: 10.64898/2026.02.25.707995

Figure Lengend Snippet: A, B. Viability of 4T1 ( A ) and CMT167 ( B ) cells after a 3-day coculture with or without AMs, in the presence of AC484 (50 nM) and/or IFNγ at indicated concentrations (ng/mL). C, D. Comparison of cytokine levels in conditioned medium collected from AMs cocultured with 4T1 ( C ) or CMT167 ( D ) tumor cells, supplemented with AC484 or DMSO controls. Cross makes indicate values beyond the detection range. Cytokines in the 13-plex panel that did not reach the detection limit in either condition were not plotted. E . Viability of 4T1 cells after coculture with or without AMs, comparing direct cell-to-cell contact versus separation by a transwell insert. F . Representative histograms of p-STAT1 (Y701) in AMs from wild-type (WT) or IFNγ knockout (KO) mice after 48 h coculture with CMT167 cells. AMs cultured alone serve as controls. G . Analysis of the fold change in p-STAT1 geometric MFI normalized to non-cocultured AMs from F. H . Viability of CMT167 cells after coculture with or without AMs harvested from WT or IFNγ KO mice, in the presence or absence of AC484. I . Representative histograms of p-STAT1 (Y701) in AMs after 24 h coculture with 4T1 tumor cells, with AC484 (100 nM) and/or the JAK inhibitor ruxolitinib (3 μM). AMs cultured alone with DMSO served as controls. J . Analysis of the fold change in p-STAT1 geometric MFI, normalized to DMSO-treated, non-cocultured control AMs, from experiments in I. K . Viability of 4T1 cells after coculture with or without AMs, in the presence or absence of AC484 (100 nM) and/or ruxolitinib (3 μM). For all bar graphs, data represent individual animals (dots), with mean ± SEM (bars). Unpaired t-test for two-group comparisons; one-way ANOVA for more than two groups: ns, p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.

Article Snippet: In experiments involving the JAK inhibitor ruxolitinib, BAL cells were pre-incubated with ruxolitinib (3 μM, MedChemExpress) in complete RPMI for 2 h, washed, and then cocultured as described, with ruxolitinib maintained throughout the assay at 3 μM.

Techniques: Comparison, Knock-Out, Cell Culture, Control

Journal: medRxiv

Article Title: Community Detection and Patient Experience Analysis in Reddit Conversations on Janus Kinase Inhibitors using Large Language Models

doi: 10.64898/2026.02.02.26345429

Figure Lengend Snippet:

Article Snippet: The main objectives of this study were to: Identify and analyze unique communities: participating in discussions regarding JAK inhibitors on Reddit.

Techniques: Labeling

Journal: medRxiv

Article Title: Community Detection and Patient Experience Analysis in Reddit Conversations on Janus Kinase Inhibitors using Large Language Models

doi: 10.64898/2026.02.02.26345429

Figure Lengend Snippet:

Article Snippet: This study demonstrates the integration of graph-based, community detection, and large language models (LLMs) to characterize discourse in Reddit about Janus kinase (JAK) inhibitors, providing a novel perspective for real-time pharmacovigilance and analysis of patient-reported experiences in online health discussions.

Techniques: Labeling